ANI Calculator

Welcome to ChunLab's online Average Nucleotide Identity (ANI) calculator.

ANI has been widely used to compare two prokaryotic genome sequences when classifying and identifying bacteria by calculating the ANI value of two prokaryotic genome sequences. Our ANI Calculator uses the OrthoANIu algorithm, an improved iteration of the original OrthoANI algorithm, which uses USEARCH instead of BLAST (Lee et al. 2015). For large data sets, please refer to the standalone OrthoANIu tool available here.

Citing this software

Yoon, S. H., Ha, S. M., Lim, J. M., Kwon, S.J. & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek. 110:1281–1286.[Full Text]
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    sequence A

    Fasta QC

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    sequence B

    Fasta QC

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    OrthoANIu Results